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The Extended 20-Day Protocol Some practitioners recommend a longer cycle for potentially enhanced benefits: Daily dose : 5-10mg Duration : 20 consecutive days Total per cycle : 100-200mg Frequency : 1-2 times per year This extended protocol may be particularly suitable for older individuals or those seeking more pronounced effects on biological aging markers

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Mitochondria were treated with pyruvate/malate/FCCP followed sequentially with rotenone, succinate, antimycin A, and N,N,N,N-Tetramethyl-p-phenylenediamine (TMPD)/ascorbic acid (n = 5)